Tag

Part:BBa_K2144009

Designed by: Oskar hman   Group: iGEM16_Stockholm   (2016-10-12)


Sequence for His6-tag and LPXTGG-tag

The His6-tag enables purification of proteins with IMAC. The LPETGG-tag enables the use of the "match making" enzyme Sortase A, a commonly used transpeptidase. The key feature of Sortase is the conjugation reaction it carries out, where the enzyme recognizes a specific amino acid sequence, a so called sorting motif (LPXTG motif in the case of S.aureus) and conjugate this sequence with another unit carrying an oligo glycine motif. The linker is added to allow for proper folding

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
None